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91 By regulating the release of glutamate, NAAG is believed to play a role in numerous neurological disorders

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Sections were incubated in rabbit anti-GFAP antibody (Abcam, catalog #ab7260, 1:1,000) and donkey anti-rabbit Alexa Fluor 488 nm (Invitrogen, catalog #A21206, 1:250), followed by Hoechst 33258 (Sigma-Aldrich, #94403, 1:15,000) for 2 min

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Finally, Gene Set Enrichment Analyses (GSEA) were performed using the fgsea v1.28.0 R package, based on average log2-fold changes obtained from the FindMarkers function with its test.use parameter set to DESeq2 on pseudobulk profiles of each cell type

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