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Immunofluorescence staining revealed significantly more P63 + cells in the GSH-treated D4 skin organoid compared to the control group (Fig

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For this purpose, two buffers with pH 1 and 4.5 were prepared, then 100 L of the supernatant solution were added to 2.5 ml of buffer with pH = 1, and the amount of absorbance at two wavelengths of 530 and 700 nm was read by spectrophotometric method, and then the same method It was also repeated with pH = 4.5

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