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For the staining procedure, the cells were first stained extracellularly, then fixed and permeabilized using BD Cytofix/Cytoperm (Cat# 554714, BD Biosciences), followed by intracellular staining

Consoli U, Priebe W, Ling YH, Mahadevia R, Perez-Soler R, Andreeff M: Annamycin, a novel anthracycline, is not affected by p-glycoprotein-related mdr: Comparison with Doxorubicin and Idarubicin

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N-acetylcysteine (NAC) is a modified form of the amino acid cysteine with an attached acetyl group that enhances its stability and absorption

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