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Fixation was carried out using BD Pharmingen Fixation Buffer (Mouse Foxp3 Buffer Set, 560409) for 30 minutes at 4C in the dark, followed by centrifugation at 500g for 5 minutes
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[DOI] [PMC free article] [PubMed] [Google Scholar] 171.Mac Nair C.E., Nickells R.W
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