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Taking 2mg or less a day of vitamin B12 in supplements is unlikely to cause any harm.

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The tissue samples were homogenised in phosphate-buffered saline (PBS, pH 7.4) containing 0.1 mM butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, Valencia, CA, USA) and sonicated for 30 s in an ultrasonic ice bath (Branson, USA)