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Genetic ablation of fas-activated serine/threonine kinase ameliorates alcoholic liver disease through modulating HuR-SIRT1 mRNA complex stability

Biochemistry, molecular biology and cell biology of gonadotropin-releasing hormone antagonists

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Kanbay, M

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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker