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Briefly, FAT protein was expressed using minimal media with M9 salts: 6.8 g/L Na 2 HPO 4 , 3.0 g/L KH 2 PO 4 , 0.5 g/L NaCl, 1 g/L 15 NH 4 Cl, 2 mM MgSO 4 , 100 M CaCl 2 , 0.4% glucose, 500 M thiamin, 0.5X MEM vitamins, and 10 M FeCl 3 6H 2 O, CuSO 4 5H 2 O, MnSO 4 H 2 O, and ZnSO 4 7H 2 O

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