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Pellets were resuspended in SCB buffer (20 mM TrisHCl, 100 mM NaCl, 0.1 mM MgSO 4 , pH 7.4) and clarified by centrifugation (10,000 rpm, 10 min, 4 C)

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Effects span from direct biochemical actions (copper delivery, antioxidant activity) to complex cellular responses (gene expression changes, differentiation effects)

Regarding the second, a key issue is the importance of changes in glutathione status that may be monitored as glutathione redox potential (thermodynamic signaling)

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