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These analyses were used to assess long-term systemic and local toxicity

Cells were lysed with DPBS solution containing 1% Triton X-100 (FUJIFILM Wako pure chemical, Osaka, Japan), and then fluorescence intensity was measured at 515 nm (excitation: 490 nm) using a SpectraMax M2e microplate reader (Molecular Devices, San Jose, CA)

Together, these findings suggest that peripheral biomarkers have the potential to become a valuable diagnostic aid across multiple forms of dementia, offering a rare blend of accessibility, biomarker specificity, and emerging commercial viability

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In comparison, the 50-mg/kg dose displayed more pronounced effects, increasing them by 103.1% and 77.8%, respectively, in contrast to the TAA group, approaching normal values (Figure 6)