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In the field of cardiovascular diseases, PAG activates adrenergic receptors, promoting high platelet reactivity and inflammatory responses, notably increasing the risks of complications such as myocardial infarction, stroke and heart failure (9598)

Ethics statement The studies involving humans were approved by The Ethics Committee of Weifang Peoples Hospital

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Differentiation and transfection of bovine intramuscular preadipocytes Bovine intramuscular preadipocytes were cultured in 6-well plates in a 5% CO incubator (Thermo Fisher Scientific) at 37 C until they reached 80% confluence

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Male SAMP8 mice develop LV hypertrophy with diastolic dysfunction as early as 6 months of age ( 2.2.3 Fischer 344 rats (H 2 FPEF score 3) The aged Fischer 344 rats develop pathophysiology of aging in various organs, including the heart (Walker et al., 2006) with evident pulmonary congestion and exercise intolerance at 25 months of age (Suen et al., 2019) by 28 months of age, these animals also develop LV systolic dysfunction and chamber dilation, which is infrequently seen in human HFpEF (Walker et al., 2006), thus limiting the use of Fischer 344 rats as an HFpEF model