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To get an objective measure of the signal generated on Western blot, a densitometer was used to scan the blot or film, and imaging software was used to compare signals
Cooke JP, Meng S

Prognosis-related molecular subtypes and immune features associated with hepatocellular carcinoma

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Metabolites were separated on a Luna 3 m NH2 100 (150 2.0 mm) column (Phenomenex)