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Cells were washed with cold PBS and resuspended in Buffer I (0.25 M sucrose, 1 mM EDTA, 20 mM HEPES, pH 7.4) containing a protease inhibitor cocktail

The peptide has not produced significant adverse effects even at doses many times higher than those used therapeutically in rodent research

However, it increased heart rate, cardiac output, and induced myocardial degeneration in mice and thus was terminated as a preclinical candidate (Yau et al., 2017)

Detection of normal cells in an early stem cell compartment in myeloid leukemia by molecular cytogenetics