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Yes, in research
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Collision energy was set at 35 eV for negative PI scan, 20 eV for positive NL scan, and 50 eV with 20 eV of collision energy spread (CES) for positive EPI scans
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In brief, the brain was homogenized in phosphate buffered saline (pH 8.0) using a homogenizer
In fact, the only study that I could find involving humans was a very small retrospective study with just 16 participants
