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Affinity purified protein samples were further resolved by size exclusion chromatography and assessed by SDS-PAGE and negative stain TEM before vitrification and cryo-EM imaging (Supplementary Fig

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Forty-eight hours after injection, animals were anesthetized and perfused intracardially with saline followed by cold 1% paraformaldehyde/1.25% glutaraldehyde

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Additional information Supplementary Information accompanies the paper on the Experimental & Molecular Medicine website Supplementary information Rights and permissions This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited

Storage assessment: If the vial has been left at room temperature for extended periods, degradation has accelerated