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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

Dynamic imaging tools, including fluorescent probes such as BODIPY-C11, offer real-time observation of lipid peroxidation in live cells, aiding in the determination of the ideal threshold for successful therapy

[DOI] [Google Scholar] Wellen K.E., Thompson C.B

10.3390/ijms18081638 116 YangT.LiangZ.WenC.JiangX.WangL

The 4mg dose delivered 15.2% weight loss (matching Wegovy) with only 18% nausea

Brain (2017) 140(7):190013