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The freeze-thaw cycle can damage the peptide's structure through the formation of ice crystals, leading to aggregation and loss of bioactivity

You'll be doing them a favor by ensuring they have the best possible opportunity of developing a healthy brain and body, and you'll be doing yourself a favor by getting enough vitamin B12

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doi: 10.1038/s41467-018-05979-8 88 YuZJiangNSuWZhuoY

de Jonge HW, Dekkers DH, Lamers JM

The thymus starts to atrophy around age 15 and continues to decline until approximately 80-90% of its activity has decreased by age 35-45, which is when the chronic diseases of aging start becoming prevalent