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After 8 h, cells were harvested in Passive Lysis Buffer (Promega Corp., Madison, WI) and the luciferase activity was measured by the Nano Dual-luciferase report assay System (Promega Corp., Madison, WI)

Regulation of metallothionein gene expression by oxidative stress and metal ions

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reported that both short- and long-term supplementation of watercress, which is rich in lipid soluble antioxidants (i.e., -tocopherol, -carotene, and xanthophyll), can reduce exhaustive exercise-associated lipid peroxidation and DNA damage (Fogarty et al., 2015)

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