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One study, for instance, notes that people who are overweight or obese appear to have lower levels of vitamin B12 than those with a body mass index (BMI) that falls within the range considered to be normal. People who took vitamin B12 supplements of their own volition gained between 2.5 and 17 fewer pounds (1.27.7 kg) over ten years compared to those who did not take any form of supplementation with this vitamin

Materials and methods Chemicals: Ferrous chloride (FeCl 2 ), ferrous sulphate (FeSO 4 .7H 2 O), sodium hydroxide (NaOH), PEI (MW 25,000) NaOH were procured from Sigma Aldrich

These observations have been associated with DNA hypomethylation, reduced expression of DNMTs, enhanced susceptibility to oxidative stress and apoptosis [37]