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These reactions can escalate quickly

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After 12 h, cells were washed with D-PBS to remove the complete medium and loaded with 40 L of 10 M coelenterazine 400a (Maokangbio) diluted in the assay buffer (HBSS containing 0.01% bovine serum albumin and 5 mM HEPES, pH 7.5) per well

Elesclomol, a Cuproptosis inducer, forms a complex with Cu(II) and preferentially accumulates in cells with high mitochondrial membrane potential, disrupting energy metabolism and activating Cuproptosis [85, 86]

CNS Neurol Disord Drug Targets (2007) 6(4):2517.10.2174/187152707781387279 87 GaspariniCFGriffithsLR

[PubMed] [Google Scholar] 2.Khavinson V.K., Linkova N.S., Tarnovskaya S.I