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Description
TSPCs in the N or NAC group were lysed in lysis buffer (50 mmol/L Tris-HCl, pH 8.0, 1 mmol/L EDTA, 1% Triton X100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 150 mmol/L NaCl) containing proteinase inhibitors (Thermo Fisher Scientific, Rockford, IL, USA)

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Ottawa Hosp Res Instit

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10.1016/j.toxrep.2022.03.019 47

Importantly, TNF- synergizes with IFN- to downregulate phosphoenolpyruvate carboxykinase 1 (PCK1) expression [72]
