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A homogenization buffer was prepared, composed of 300 mM saccharose, 10 mM K-HEPES, and 1 mM EGTA, which were diluted in ultrapure water

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The ALF model group received the same amount of isotonic saline

Once cysteine loses its prized electron during all this commotion, it becomes a free radical and neutralizes itself by butting heads with another cysteine thats been through the same ordeal

Nature 547 , 453457 (2017)

This might happen because ROS is removed without enzymes by boosting the effects of non-enzymatic antioxidants or by increasing the activity of other antioxidant enzymes 108