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The cells were pelleted by centrifugation and lysed by sonication in 25 mM Tris pH 7.5, 500 mM NaCl, supplemented with 1 mM phenylmethylsulfonyl fluoride and complete (EDTA-free) protease inhibitor tablets (1 tablet per 40 mL of sonication buffer, Roche)

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Also, this kind of plasma treatment can effectively reduce the concentrations of mycotoxins in date palms (Gavahian & Cullen 2020)