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Optional: If the lysate is too viscous, add RNase A (10 g/mL) and DNase I (5 g/mL) and incubate on ice for 10-15 minutes

Most infusions take between 3045 minutes

With the expansion of agricultural production scale, the application of pesticides has shown a sharp upward trend

10.1016/j.jff.2017.05.056 [DOI] [Google Scholar] 26.Hayasaka N, Shimizu N, Komoda T, Mohri S, Tsushida T, Eitsuka T, et al

doi: 10.3147/jsfp.29.149

Errors in co- or post-translational protein modifications Various protein modifications that occur during or after protein synthesis (co- or post-translational), such as cofactor incorporation, glycosylation, lipidation, and other modifications, affect the folding and conformation of many proteins