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doi: 10.1073/pnas.0800121105 16 PekarskyYBalattiVCroceCM

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A.DhaneshwarS

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Subsequent analysis found that the trace mineral, selenium, in the form of the amino acid, Sec, is essential for the activity of GPx-1 (127, 203, 309, 312)

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Total protein content was estimated by the Bradford method using bovine serum albumin as a standard

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Absorbance at 450 nm was measured by a microplate spectrophotometer (Bio-Rad)

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