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Apoptotic caspases are categorized as initiator caspases (caspase-8, caspase-9, and caspase-10) and effector caspases (caspase-3, caspase-6, and caspase-7) (Man and Kanneganti 2016)

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New insights into the roles of Irisin in diabetic cardiomyopathy and vascular diseases

We used a temperature of 35 C as a compromise between yeast growth and enzyme optima and a WIS content of 5%, implying an inhibitor concentration that is likely to be tolerated by all the strains

Relative gene expression was calculated using the 2 Ct method, normalizing to GAPDH as an internal control

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