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In addition, the NKT cells in MB patients were present and functional, suggesting the possibility of using these NKT cells to kill MB cells at least in a subset of patients (173)

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To prepare the Transwell assay, murine melanoma cells (1 10 5 cells per insert) cultured in 200 l RPMI1640 medium supplemented with 0.5% FBS were plated on the upper insert (6.5 mm diameter transwell with 8 m pores, Corning), while 800 l RPMI1640 medium supplemented with 0.5% FBS, with or without hHGF or dimeric macrocycle was added to the bottom chamber

Furthermore, we treated dividing and senescent cells with 20 M of CPP-CAND and assessed caspase 3/7 activity to examine whether the apoptosis observed was caspase-dependent

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it made me feel less than human