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The native host-independent paradigm to natural product identification, pathway characterization, and enzyme discovery is where synthetic biology has played the most prominent role

PubMed Central , PLOS ONE , vol

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[DOI] [PubMed] [Google Scholar] 25.Sechi G

FAD absorption spectroscopy To assess the presence of protein-bound FAD, 100 g of recombinant WT or D285N mutant FSP1 protein or 10 g of free FAD control was resuspended in a total volume of 200 l in 50 mM sodium phosphate pH 7.4
