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Description
Mitochondria were treated with pyruvate/malate/NADH followed sequentially with rotenone/antimycin A, TMPD/ascorbic acid, and azide (n = 6)
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10.3389/fmicb.2021.780768 Front Microbiol
What changes is that the reconstituted solution now contains both active ingredients at their respective concentrations
The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and is easily adaptable for automation without a separation step
