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In Vitro Pull-Down Binding Assays To determine whether GSTP binds directly to HMGB1, recombinant polyhistidine-tagged GSTP (WT), GSTP (S184A), GSTP (S184D), and HMGB1 proteins were expressed in Escherichia coli BL21 (DE3) and purified through Ni-NTA spin column (Qiagen)

The chloroform extract of P

Overexpression of P-gp is a common occurrence in 50% of cancer cells [35]

We performed at least technical duplicates for each experiment and used hippocampal neurons originating from different animals

& Chu, X

Fifteen of the proteins we identify associated with cancer risk, including SFTPB and GAS1 , did not appear to be under active current investigation as a drug target (Fig