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The Nde I restriction site upstream of torA was then removed by QuickChange mutagenesis (Agilent, Waldbronn, Germany) according to the manufacturers protocol using primers QC- Nde I-fw and QC- Nde I-rv (Supplementary table S3) resulting in the pPT plasmid

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Parp mutations protect against mitochondrial dysfunction and neurodegeneration in a PARKIN model of Parkinsons disease