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Experimental data suggest that microbes such as p ks (polyketide synthase)+ Escherichia coli (pks+Ec), ETBF , Fn , and SGG , damage the gut-barrier lining and colonocyte DNA, increase proinflammatory cytokines and oxidative factors, and produce potentially carcinogenic toxins (17, 24, 5861)

Next, the samples were reduced and alkylated by adding 20 mM iodoacetamide (IAA) and incubated for 1 h in the dark, followed by a 1 h incubation with 20 mM DTT to eliminate residual IAA

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[DOI] [PMC free article] [PubMed] [Google Scholar] 55.Rahban M., Joushi S., Bashiri H., Saso L., Sheibani V