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no preservative means higher contamination risk Normal Saline (0.9% NaCl): Acceptable alternative

So a 5 mg vial contains 5,000 mcg, and a 250 mcg dose is 0.25 mg

Reconstitution (research only): Prepare each vial separately using sterile bacteriostatic water according to lab protocol

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Signs of intramuscular injection include deeper, more intense pain during injection, increased bruising, faster onset of side effects (nausea, headache appearing within hours instead of the typical timeline), and a sore, tender feeling in the thigh muscle for a day or more after injection

These processes help the body respond to strain, injury, and physical stress affecting muscles, tendons, ligaments, and joints