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Science 323, 12111215
They all face the same problem: mental fatigue kills performance
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After incubation, cells were washed thrice with PBS, co-stained with NucBlue and fixed with 4% PFA
The first approach relies on the physicochemical properties of the LNP, including lipid composition as well as the preparation method, size and surface charge which can alter in vivo biodistribution ( The ability of CD4+ T cells to undergo receptor-mediated endocytosis, even in a resting state, highlights a potential role for active targeting to ensure efficient Tat uptake
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