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Some newer ER formulations produce therapeutic effects by the MPH dose being delivered initially by a bolus that achieves peak plasma concentrations in early morning within 2 hours of dosing, and a second extended and controlled delivery of the drug that achieves the required plasma concentrations in the afternoon (Swanson et al ., 2002[119], 2003[120]

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The formazan that was formed was dissolved in DMSO for 30 min on a shaker, and absorbance was measured using a spectrophotometer at a wavelength of 570 nm