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Brains were removed, post-fixed in the same fixative overnight at 4C, and cryoprotected in 20% (w/v) sucrose (Sigma) overnight followed by 30% (w/v) sucrose overnight, embedded in OCT medium and stored at -80C until use

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In the gut, the microbiota metabolizes BAs and other dietary nutrients, which flow back to the liver via the portal vein 4

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