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It's attracted growing interest in research circles because of how directly it targets a specific enzymatic pathway - which makes it a useful tool for studying how NNMT activity influences downstream metabolic signalling at the cellular level

Advanced Drug Delivery Reviews, 110 , 312

Briefly, 3 10 4 cells in 100 l serum-free medium were added to the upper compartment of the chamber, while the lower compartment was filled with 600 L of regular culture medium, then the cells were incubated at 37 C for 36 h
