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In constraint-based modeling, cellular metabolism is represented as a network of reactions with constraints based on known biochemical principles to predict metabolic fluxes and identify active pathways

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Secondly, the redox status of the cell affects many of the enzymes in one-carbon metabolism including MATI, MATIII, MS, BHMT, as well as CBS and GCS in the transsulfuration pathway, and therefore one cannot evaluate GSH metabolism without including the methionine and folate cycles

Engineering strategies can generate functionally enhanced exosomes, reducing dependence on native complex cargo and mitigating heterogeneity-related uncertainty

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The mechanisms involved include mitochondrial and endoplasmic reticulum stress, leading to oocyte apoptosis and autophagy, disruption of the cytoskeletal structure, and a reduction in the extrusion rate of the PB1 (Zhai et al., 2018