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Reduced MTHFR activity has been associated with altered methylation capacity and elevated homocysteine metabolism, which may contribute to fatigue and neurocognitive symptoms in susceptible individuals (9,10)

10.1046/j.1365-2222.1996.d01-279.x [DOI] [PubMed] [Google Scholar] 61.Speeckaert R, Dugardin J, Lambert J, Lapeere H, Verhaeghe E, Speeckaert MM, et al

Microbial riboflavin biosynthesis associates with response to dietary fiber consumption: time to personalize adjunct therapy in patients with inflammatory bowel disease

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All steps were the same as for the 96-well plate assay except that the culture volume was 1 mL, the volumes of the fixative, staining solution, and acetic acid were scaled proportionally (0.5 mL fixative, 1.25 mL of crystal violet solution, and 1.25 mL of glacial acetic acid per tube), and the washes were performed by gentle pipetting (2 mL water per tube per wash) instead of by submersion

FEBS J 275(6):10801088