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The protocol for handling it depends on how much time has passed since the missed injection
However, the enhanced cellular complexity of an ex vivo system comes with endogenous production of cytokines, a process demonstrated in previous slice culture experimentation (Dionne et al., 2011
Do not look down at it from above or up at it from below

When reconstituted with bacteriostatic water and stored properly in a refrigerator (not frozen), PT-141 is typically stable and potent for 30 to 60 days

Evoked patch pipettes (47 M resistance) were filled with an internal solution containing: 120 mM potassium gluconate, 10 mM KCl, 1 mM MgCl 2 , 0.5 mM EGTA, 40 mM HEPES, and adjusted to pH 7.2 with KOH)

Cycling protocols help minimize copper accumulation concerns
