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Sequence alignment analysis of SRD5As across different species, including the plant homolog DET2 involved in the synthesis of phytohormones 41 , indicated that while the residues involved in the binding of NADP are highly conserved, the residues in the DHF-binding pocket are much less conserved (Extended Data Fig

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doi: 10.1016/j.jss.2017.02.061

After 5 min, 2 mL of 10% AlCl was added, followed by another 5 min of incubation