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In brief, four parts of neat methanol were added to each sample and vortexed, one part of chloroform was then added to the sample and vortexed, and finally three parts of water were added to the sample and vortexed

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When death ligands such as TNF (tumor necrosis factor-alpha), Fas ligand (FasL), or TRAIL bind to their corresponding cell surface receptorsTNFR1, Fas, and death receptors 4 and 5 (DR4/5)it triggers a signaling cascade