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Chemical Labeling Using HaloTag Technology Labeling is a two-step process, where amine-reactive HaloTag Succinimidyl Ester (O4) Ligand reacts with primary amines on lysine amino acids on the antibodies and proteins

doi: 10.1007/s40005-016-0256-x

A study from Denmark confirms this

Curing the brain: in search for new astrocyte-specific therapies

Dixon S.J., Lemberg K.M., Lamprecht M.R., Skouta R., Zaitsev E.M., Gleason C.E., Patel D.N., Bauer A.J., Cantley A.M., Yang W.S., et al

Add 1 mL of Reagent I (the proportion of tissue and reagents can be kept constant), fully grinding on ice (using liquid nitrogen will have a better grinding effect)
