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Phase separation of C9orf72 dipeptide repeats perturbs stress granule dynamics

By studying the molecular mysteries of these remarkably resilient creatures, researchers are creating breakthrough tools to combat human disease and advance biotechnology

The dipole character of alpha-helices, for instance, lowers the pK a value and renders cysteine residues located towards the N-terminal end in general more reactive than cysteines positioned towards the C-terminal end [25]

Briefly, cell pellets or snap-frozen tissues were homogenized in a lysis buffer containing 10 mM Tris-HCl, pH 6.87.5, 2 mM EDTA, 0.5% SDS, and freshly added 2-mercaptoethanol