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G., & Joosten, L

Cells grown on 1.5 circular glass coverslips were washed with ice-cold 1X PBS and fixed in 4% paraformaldehyde for 10 min at room temperature 38

Shaheen LA, Liberman MC

The plate was then incubated for 5 min at room temperature before measuring the absorption at 590 nm using the Fluostar Optima (BMG LabTech, Ortenberg, Germany)

Despite the pineal gland being the main source for melatonin, extra pineal sources share in its synthesis, such as the retina, GIT mucosa, kidney, adrenals, thyroid, pancreas, and platelets, and other brain regions including the hypothalamus, pons, medulla, cerebellum, and cerebral cortex [151]

In fact, NNMT levels vary between the stroma and cancer cells and patients with high stromal NNMT tend to have a poor prognosis (38, 43, 62)
