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A more comprehensive strategy to avoid GSH-based platinum drug resistance mechanisms is based on suppression of GSH synthesis in the malignant tissue, which wouldbesides GST-catalyzed drug conjugation in cancer cellsalso target extracellular oxaliplatin inactivation via GSH adduct formation 20

PMID: 20940632 Rihn, J.A., Yang, N., Fisher, C., Saravanja, D., Smith, H., Morrison, W.B., Harrop, J., Vaccaro A.R..: Using magnetic resonance imaging to accurately assess injury to the posterior ligamentous complex of the spine: A prospective comparison of the surgeon and radiologist

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Establishment of cell co-culture system and tubing formation assay The HUVEC cell line was from the cell bank in our lab (Jilin Provincial Key Laboratory of Antler Biology), and cultured in DMEM medium supplemented with 100 U/ml penicillin, 100 g/ml streptomycin, and 10% fetal bovine serum (HyClone, Beijing, China), in a humidified incubator with 5% (v/v) CO 2 atmosphere at 37 C

Muscle cramps can last anywhere from a few seconds to several minutes and may be caused by dehydration, overuse, or nutritional deficiencies, especially a lack of essential vitamins and minerals that support muscle function