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Through this process, cationic NPs bypass the cell surface lipid bilayer and enter acidic vesicles, where their amino groups sequester protons supplied by the proton pump, v-ATPase

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Dicer-substrate siRNA (DsiRNA) is composed of a sense strand with tetraloop hairpin, a constant sequence, and a 2123 nt antisense strand complementing the target mRNA

For best laboratory handling, we recommend preparing a minimum total volume of 5 ml after reconstitution to reduce the chance of gelling