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Cell pellets were resuspended in ice-cold buffer (2530 mL/L) containing 150 mM NaCl, 20 mM Tris (pH 8.0) and 1X Protease Inhibitors Cocktail (Roche)

183 In about 50% of cases diffuse traumatic brain injury is complicated by long-term neurological consequences including cognitive decline, sleep disturbances and depression

Avoid freezing peptides, as this can damage their structure

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Further, this study explores the significance of glutathione (GSH) and phytochelatins (PCs) and a series of candidate genes that showed potential role in HMs stress tolerance in diverse plant species

Bonan NB, Steiner TM, Kuntsevich V, Virz GM, Azevedo M, Nakao LS, et al