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Protein measurement Total lung tissue protein was extracted using RIPA tissue protein lysate (Solarbio, Beijing, China)

The resulting cDNA plus oligo-dT was used as template for qPCR, in a final volume of 10 L containing SYBR Green PCRMasterMix (Thermo Fischer Scientific, 4309155) and 5 M of the following primers: -actin (forward): 5-GGCTGTATTCCCCTCCATCG-3

The extremely slow and variable activity of dihydrofolate reductase in human liver

Wide annealing temperature window

Furthermore, no significant changes in the levels of other markers were observed

Fine-tuning Further training of pretrained, often general-purpose models using a smaller, specialized dataset to adapt it for a specific application