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The design of the linker sequences takes into consideration the rigidity of the helical structure, the various spacing and relative orientations of MBP and target proteins connected by different helical linkers, and the minimal structural perturbation by a helical linker that connects the C-terminal helix from MBP with the N-terminal helix from a death domain superfamily member

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A total of three types of benzoic acid derivatives are employed as MALDI matrices: lithium 2,5-dihydroxybenzoate (LiDHB), alkylated trihydroxyacetophenone (ATHAP), and COOH-NHMe(IV) (Figure )