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Statistical Analysis For the cell-based experiments, all statistical calculations were performed using unpaired t-tests for protein synthesis and signaling, comparing each group to the control group

Secondly, the intestinal metabolism of L-carnitine is thought to generate trimethylamine-N-oxide (TMAO), a compound that has been linked with faster atherosclerosis progression

Samples were analysed using a flow cytometry analyser (FACS LSR II SORP or Fortessa X20, BD Biosciences) at a rate of less than 7,500 events per second

After treatment, 78 patients received 2 grams daily of a branded P

FIGURE 4 Surprisingly, cascades with TMLH, but lacking one or more (active) enzymes (combination C, D, E, F, and G) also showed L-carnitine biosensor signal output, albeit it was significantly lower than that of the complete cascade (combination H)